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Confocal Microscopy

The optic microscope is an instrument which uses the light as energy source to observe small objects magnified. It has been the first modern scientific instrument and deeply influenced the scientific knowledge and the culture. The history of the microscopy  is deeply linked to that of histology and anatomy.

This is particularly true for the sciences of the nervous system, in which the contributions of the microscope to the physiology and pathology of the nervous system have been fundamental.

The confocal microscopy unit has been created to provide support for scientists who wish to implement confocal techniques in their research programs. The confocal microscopy unit is composed by two confocal systems, one fluorescence microscope and three softwares for image analysis.

One inverted confocal microscope: Leica SP5 (EBRI-CNR-S. Lucia Foundation), equipped with four fluorescence channels plus a transmitted light channel.

One upright confocal microscope: Nikon C1 (EBRI), three fluorescence channels plus a camera for widefield imaging. In addition an inverted fluorescence microscope (EBRI) equipped with a camera for image acquisition.

An incubation chamber for microscopes allow to perform live imaging experiments.

For image analysis and processing three softwares are available: Image J, LUCIA (Nikon) and Imaris from (BitPlane).

An histology room is available for preparation of  fluorescent and immunoperoxidase slides of both tissue and cells.

The confocal microscopes are equipped to perform routine imaging on fixed and live cells as well as biophysical analytical techniques such as Förster resonance energy transfer (FRET) and fluorescence recovery after photobleaching (FRAP) microscopy.

The  confocal microscopy unit is open to all researches working at the institute: we train users to drive instrumentation independently or weoffer personalized assistance.

We also offer users comprehensive training (data acquisition and image analysis), assistance in the design of experimental protocols and ongoing consultation and support.