Caricamento
Ebri.it



Genomics

The EBRI Genomics Facility provides resources to investigate gene expression and functions with a focus on applying RNA/DNA microarray technologies.

The Facility combines state of the art technology and expertise in genomics and bioinformatics.

Probe intensities in a 2-color microarray study from a chip with 44000 probes, where each probe corresponds to a coding mRNA
Probe intensities in a 2-color microarray study from a chip with 44000 probes, where each probe corresponds to a coding mRNA

The aim of this project is to provide new knowledge on molecules that act at the beginning of neurodegenerative phenomena, focusing on synaptic functions.The microarray technology of the EBRI Facility is based on the Agilent platform, that provides high-density microarrays chips with 60-mer oligos synthesized in-situ, allowing the analysis of gene expression profiles, mRNA and microRNA, genomic aberrations on DNA samples (CGH, Comparative Genomic Hybridization), methylation and promoter profiles of DNA (ChIP-on-chip).

Probe intensities in a 2-color microarray study from a chip with 44000 probes, where each probe corresponds to a coding mRNA.

Heterozygous 0.3 Mb DNA deletion from a patient affected by mental retardation, from a 2-color microarray CGH study. Each dot corresponds to the signal of  a 5 kb DNA segment.

 

Heterozygous DNA

Heterozygous 0.3 Mb DNA deletion from a patient affected by mental retardation, from a 2-color microarray CGH study. Each dot corresponds to the signal of  a 5 kb DNA segment

Project currently ongoing at EBRI Facility in collaboration with Prof. Cattaneo's group:

  • - Alzheimer’s Disease project: mRNA and microRNA gene expression profiles in animal models and human samples of Alzheimer’s Disease for the identification of molecular changes and biomarkers for an early disease diagnosis.
    - microRNA, synapses and neurodegeneration.
  • - Role of early inflammation in Alzheimer’s disease.
    - Spinocerebellar Ataxia: for identification of high-density genomic aberration profiles in the DNA sequence of specific genes (in collaboration with Dr. Veneziano).
  • - Data mining techniques  for analysis of genomics and clinical dataset.